Molecular cloing and bioinformatics analysis of lactatedehydrogenase from
Taenia multiceps
Cheng Guo, Yu Wang, Xing Huang, Ning Wang, Ming Yan, Ran He,Xiaobin Gu, Yue Xie, Weimin Lai,
Bo Jing, Xuerong Peng, Guangyou Yang
Abstract
Coenurus cerebralis, the larval stage (metacestode or coenurus) ofTaenia multiceps, parasitizes sheep, goats, and other ruminants and causes coenurosis. In this study, we isolated and characterized complementary DNAs that encode lactate dehydrogenase A (Tm-LDHA) and B (Tm-LDHB) from the transcriptome ofT. multicepsand expressed recombinant TmLDHB (rTm-LDHB) inEscherichia coli.Bioinformatic analysis showed that both Tm-LDH genes (LDHA and LDHB) contain a 996-bp open reading frame and encode a protein of 331 amino acids. After determination of the immunogenicity of the recombinant Tm -LDHB, an indirect enzyme-linked immunosorbent assay (ELISA) was developed for preliminary evaluation of the serodiagnostic potential of rTm-LDHB in goats.However, the rTm-LDHB-based indirect ELISA developed here exhibited specificity of only 71.42% (10/14) and sensitivity of 1:3200 in detection of goats infected withT. multicepsin the field. This study is the first to describe LDHA and LDHB of T. multiceps; meanwhile, our results indicate that rTm-LDHB is not a specific antigen candidate for immunodiagnosis of T. multicepsinfection in goats.
copyright:©The Author(s) 2017.
Parasitology Research,(2017)116:2845-2852. DOI:10.1007/s00436-017-5568-x
Read Full Text: www.ncbi.nlm.nih.gov/pmc/articles/PMC5599447/
