Expression and characterisation ofaSarcoptes scabieiprotein tyrosinekinase as a
potential antigen forscabies diagnosis
Nengxing Shen, Ran He, Yuqing Liang, Jing Xu, Manli He, Yongjun Ren, Xiaobin Gu,Weimin Lai,Yue Xie,
Xuerong Peng & GuangyouYang
Abstract
Scabies is a disease that harms humans and other animals that is caused by the itch miteSarcoptesscabieiburrowing into the stratum corneum of the skin. In the early stages of scabies, symptoms are often subclinical and there are no efective diagnostic methods. Herein, we cloned, expressed and characterised anS. scabieiprotein tyrosine kinase (SsPTK) and evaluated its diagnostic value as a recombinant antigen in rabbit during the early stages ofSarcoptesinfestation. The SsPTK protein is ~30 kDa, lacks a signal peptide, and shares high homology with a PTK from the rabbit ear mite Psoroptes ovis cuniculi. The protein was widely distributed at the front end of mites, particularly in the chewing mouthparts and legs. Indirect ELISA using recombinant SsPTK showed good diagnostic value,with 95.2% (40/42) sensitivity and 94.1% (48/51) specifcity for detecting anti-PTK antibody in serumsamples from naturally-infested rabbits. More importantly, PTK ELISA could diagnose infection in theearly stages (infestation for 1 week) with an accuracy of 100% (24/24). SsPTK therefore shows potentialas a sensitive antigen for the early diagnosis of parasitic mite infestation.
copyright:© The Author(s) 2017.
Scientific Reports,2017,7(1): 9639. DOI:10.1038/s41598-017-10326-w
Read Full Text: www.ncbi.nlm.nih.gov/pmc/articles/PMC5575040/
