Expression, Tissue Localization and Serodiagnostic Potential of Echinococcus granulosus Leucine Aminopeptidase
Maodi Wu, Min Yan, Jing Xu, Yuqing Liang, Xiaobin Gu, Yue Xie , Bo Jing ,
Weimin Lai, Xuerong Peng and Guangyou Yang
Abstract
Echinococcus granulosusis the causative agent of cystic echinococcosis (CE), a widespread parasitic zoonosis. Leucine aminopeptidases (LAPs) of the M17 peptidase family have important functions in regulating the balance of catabolism and anabolism, cell maintenance, growth and defense. In this study, we presented a bioinformatic characterization and experimentally determined the tissue distribution characteristics ofE. granulosusLAP (Eg-LAP), and explored its potential value for diagnosis of CE in sheep based on indirect ELISA. Through fluorescence immunohistochemistry, we found that Eg-LAP was present in the tegument and hooks of PSCs, the whole germinal layer and adult worm parenchymatous tissue. Western blotting results revealed that the recombinant protein could be identified usingE. granulosus-infected sheep serum. The diagnostic value of this recombinant protein was assessed by indirect ELISA, and compared with indirect ELISA based on hydatid fluid antigen. The sensitivity and specificity rEgLAP-ELISA were 95.8% (23/24) and 79.09% (87/110), respectively, while using hydatid fluid as antigen showed the values 41.7% (10/24) and 65.45% (72/110). This is the first report concerning leucine aminopeptidase fromE. granulosus, and the results showed that Eg-LAP belong to M17 peptidase families, and that it is involved in important biological function ofE. granulosus. Furthermore, rEg-LAP is appropriate for diagnosing and monitoring CE in sheep in field. Development of a rapid test using rEg-LAP to diagnose sheep CE deserves further study.
copyright:©2018 by the authors.
Int. J. Mol. Sci. 2018, 19(4), 1063. doi:10.3390/ijms19041063
Read Full Text: http://www.mdpi.com/1422-0067/19/4/1063
