Molecular Characterization of Annexin B2, B3 and B12 inTaenia multiceps
Cheng Guo, Yue Xie, Yuchen Liu, Ning Wang, Jiafei Zhan, Xuan Zhou, Christiana Angel,
Xiaobin Gu, Weimin Lai, Xuerong Pengand Guangyou Yang
Abstract
Coenurus cerebralis, the metacestode ofTaenia multiceps, causes coenurosis, a disease severely affecting goat, sheep, cattle and yak farming and resulting in huge economic losses annually. Annexins bind calcium ions and play an important role in flatworm parasite development. To explore potential functions of annexins inT. multiceps, three homologous genes, namely,TmAnxB2,TmAnxB3 andTmAnxB12, were screened from the transcriptome dataset, amplified fromC. cerebralis cDNA and subjected to bioinformatics analysis. Then, polyclonal antibodies recognizing the recombinant TmAnxB2 (rTmAnxB2) and rTmAnxB3 were prepared for localization of TmAnxB2 and TmAnxB3 in different tissues and developmental stages by immunofluorescence. The transcription of all three genes was also measured by relative fluorescent quantitative PCR. The sizes of rTmAnxB2, rTmAnxB3 and rTmAnxB12 were 58.00, 53.06 and 53.51 kDa, respectively, and rTmAnxB12 was unstable. Both rTmAnxB2 and rTmAnxB3 were recognized by goat-positiveT. multiceps sera in Western blots. Immunofluorescence revealed that TmAnxB2 and TmAnxB3 were localized in the protoscolex and cyst wall and TmAnxB3 was also detected in adult cortex. TmAnxB2 and TmAnxB12 mRNA levels were determined to be highest in oncospheres and protoscolex, whereas transcription of TmAnxB3 was highest in scolex and immature segments. Taken together, these findings indicate that TmAnxB2 and TmAnxB12 may play critical roles inT. multiceps larvae, while TmAnxB3 may have important functions in adults. These results will lay the foundation for functional research of annexins inT. multiceps.
copyright:© 2018 by the authors.
Genes2018,9, 559. DOI:10.3390/genes9110559
Read Full Text: http://www.mdpi.com/resolver?pii=genes9110559
