Transcriptome-microRNA analysis ofSarcoptes scabieiand host immune response
Ran He, Xiaobin Gu, Weimin Lai, Xuerong Peng, Guangyou Yang
Abstract
Scabies is a parasitic disease, caused by the mite Sarcoptes scabiei,and is considered one of the top 50 epidemic diseases and one the most common human skin disease, worldwide. Allergic dermatitis, including an intense itch, is a common symptom, however diagnosis is difficult and there is currently no effective vaccine. The goal of this study was to examine the immune interaction mechanism of bothS. scabiei and infected hosts. mRNA-seq and microRNA-seq were conducted on theS. scabiei mite and on infected and uninfected hosts. We focused on differential expression of unigenes and microRNAs, as well as the real targets of unigenes in enriched immune signaling pathways.S. scabiei enhanced host immune function and decreased metabolism after infection, while the immune response of the host inhibitedS. scabiei proliferation and metabolism signaling pathways. Differentially expressed unigenes ofS. scabiei were enriched in the JAK-STAT signaling pathway and the Toll-like receptor signaling pathway. The differential expression analysis indicated that microRNAs ofS. scabiei and hosts have major roles in regulating immune interactions between parasites and hosts.
Copyright© 2017 He et al.
PLoS ONE,2017, 12(5): e0177733
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