Molecular identification and characterization of prohibitin fromEchinococcus granulosus
Xiuqin Zhong & Xingju Song & Ning Wang & Dandan Hu & Tinayu Liu & Tao Wang &Xiaobin Gu & Weimin Lai
& Xuerong Peng & Guangyou Yang
Abstract
Prohibitin (PHB) is a widely distributed protein that functions as a molecular chaperone, is involved in the regulation of cell cycle, and maintains mitochondrial structure and functions of the anti-apoptosis, senescence, and proliferation. The aim of this study was to characterize PHB in Echinococcus granulosus (EgPHB), a harmful cestode parasite of humans, many livestock species, and wild animals. We found that EgPHB is a conserved SPFH (stomatin, prohibitin, flotillin, and HflK/C) domain-containing protein, consisting of 289 amino acids, which shares 42.66-99.31 % identity with PHBs from other parasites and mammals. EgPHB was located mainly in the tegument issue of protoscoleces, in the inner body of adult worms, and was expressed widely in the germinal layer. This is the first report on prohibitin from E. granulosus, and EgPHB is considered to be a valuable protein to study more in the future.
Keywords
Prohibitin,Echinococcus granulosus, Western blotting, Immunohistochemical localization
Parasitol Res. (2016) 115:897 – 902
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