Sequence analysis of Bs-Ag2 gene fromBaylisascaris schroederiof giant panda and evaluation of the efficacy of a recombinant Bs-Ag2 antigen in mice
He G, Wang T, Yang G, Fei Y, Zhang Z, Wang C, Yang Z, Lan J, Luo L, Liu L.
Abstract
ThegeneofBs-Ag2fromBaylisascarisschroederiwas amplified, cloned and sequenced.Sequence analysisindicated that the nucleotide sequences of theBs-Ag2from adult, L2 and L3 ofB.schroederiwere completely identical. A homology search performed by BLAST revealed thatBs-Ag2shared the highest amino acidsequenceidentity with As16 protein from Ascaris suum (94%). TherecombinantBs-Ag2proteins can be successfully expressed in Escherichia coli BL21 (DE3). The rBs-Ag2 was used to evaluate their ability to induce immune protective responses in BALB/cmiceagainst L3-challenge infection in a mouse-B.schroederimodel. There was a 63.66% reduction (P<0 .001) of recovery of larvae compared with that in the control group. specific anti-bs-ag2antibodies from immune protectedmicehad significantly higher levels of immunoglobulin g (igg) (p<0.0001). our data supported the use ofbs-ag2as a potential candidate for vaccination againstB.schroederiinfection.
Copyright © 2009 Published by Elsevier Ltd. All rights reserved.
Vaccine. 2009 May 14;27(22):3007-11. doi: 10.1016/j.vaccine.2009.02.077. Epub 2009 Mar 9.
Read full text:http://www.sciencedirect.com/science/article/pii/S0264410X09003430
